Additional Selective and Differential Cell Culture Media
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NEOGEN Harlequin E. coli/Coliform Agar, 500 g
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E. coli / Coliform Agar is for the simultaneous enumeration of Escherichia coli and coliforms in food and environmental samples and is not intended for use in the diagnosis of disease or other conditions in humans.This dual chromogenic substrate medium makes the different colony types easy to distinguish allowing rapid counting of both E. coli and coliforms on a single agar.Based upon the formulation of Tryptone Bile Agar, the medium has been modified by the addition of two chromogenic substrates, one to detect the ß-glucuronidase enzyme (X- glucuronide) and another to detect the ß-galactosidase enzyme (magenta-ß-gal). Typical E. coli strains possess both enzymes but only cleave the X-glucuronide substrate, thereby producing blue-green colonies. Typical coliforms, however, possess only the ß-galactosidase enzyme and produce rose-pink colonies.For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-e-coli-coliform-agar
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NEOGEN Bile Salts No. 3, 500 g
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Bile Salts No. 3 is a mixture of bile salts for use in preparing microbiological culture media in a laboratory setting. Several media containing Bile Salts No. 3 are recommended in standard methods for multiple applications. Bile Salts No. 3 is not intended for use in the diagnosis of disease or other conditions in humans.Bile Salts No. 3 is a refined bile salts mixture, comprising mainly sodium cholate and sodium desoxycholate. Its primary use is as a selective agent for the isolation of Gram-negative micro-organisms, inhibiting Gram-positive cocci. Bile Salts No. 3 is prepared especially for use in MacConkey Agar and Violet Red Bile Agar. This fraction of bile is highly active allowing maximum selection of organisms of enteric origin at relatively low concentrations (0.15%) and is soluble in distilled or deionized water and neutral to slightly alkaline in pH.For more information visit https://www.neogen.com/solutions/Microbiology/bile-salts-number-3
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NEOGEN Dermatophyte Test Medium (DTM), 500 g
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Dermatophyte Test Medium is used for the selective isolation of dermatophytic fungi and is not intended for use in the diagnosis of disease or other conditions in humans.In 1969, Taplin et al. developed this medium for the isolation and recognition of dermatophytic fungi, the causative agent of ringworm from hair, nails, and skin. Dermatophyte Test Medium is preferred for isolation and early recognition of Microsporum, Trichophyton, and Epidermophyton genera because of a distinct color change in the medium. Rapidly-growing species may produce a complete color change in the medium in 3 days. The slower-growing species will change the indicator in longer time periods. Other organisms may grow but can be recognized as non-dermatophytes by lack of a color change.For more information visit https://www.neogen.com/solutions/Microbiology/dermatophyte-test-medium-dtm
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NEOGEN Cronobacter Selective Broth, 500 g
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Cronobacter Selective Broth (CSB) is recommended by ISO 22964 for the isolation and identification of Cronobacter spp. (formerly Enterobacter sakazakii), and is not intended for use in the diagnosis of disease or other conditions in humans.Although rarely causing infections in immunocompetent adults, members of the Cronobacter genus have been implicated in sepsis, meningitis and necrotizing enterocolitis with a high death rate in neonates. These opportunistic pathogens are common in the environment and their ability to survive desiccation presents a significant risk for post-pasteurization contamination and survival in spray dried milk products. Enzymatic digest of animal tissues and meat extract provide a source of nitrogen and essential vitamins.For more information visit https://www.neogen.com/solutions/Microbiology/cronobacter-selective-broth
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NEOGEN Tetrathionate (TT) Broth Base, Hajna, 500 g
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Tetrathionate (TT) Broth Base, Hajna is used as a selective enrichment for the cultivation of Salmonella species (except S. typhi) from samples in a laboratory setting. Tetrathionate (TT) Broth Base, Hajna is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base, Hajna, is recommended in the Compendium of Methods for the Microbiological Examination of Foods and the FDA/BAM for the Environmental Sampling and Detection of Salmonella Enteritidis (SE) in Poultry Houses and whole eggs.. Salmonella organisms may be present but injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers. Numerous reported cases of Salmonella enteritidis (SE) infections are associated with contaminated shell eggs.For more information visit https://www.neogen.com/solutions/Microbiology/tetrathionate-tt-broth-base-hajna
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NEOGEN Modified Buffered Peptone Water with Pyruvate, 500 g
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Modified Buffered Peptone Water with Pyruvate (mBPWp) is used for the isolation of Enterohemorrhagic E. coli (EHEC) in a laboratory setting. Modified Buffered Peptone Water with Pyruvate (mBPWp) is not intended for use in the diagnosis of disease or other conditions in humans. Enterohemorrhagic E. coli (EHEC) are recognized as the primary cause of hemorrhagic colitis (HC) or bloody diarrhea. This infection can also lead to hemolytic uremic syndrome (HUS). Enterohemorrhagic infections are usually food or water borne and have been implicated in undercooked ground beef, raw milk, lunchmeat, water, unpasteurized apple cider, sprouts and vegetables. Modified Buffered Peptone Water with Pyruvate is recommended in the sample preparation for the screening method following FDA/BAM Enterohemorrhagic E. coli (EHEC) Protocol.For more information visit https://www.neogen.com/solutions/Microbiology/modified-buffered-peptone-water-with-pyruvate
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NEOGEN Xylose Lysine Deoxycholate (XLD) Agar (ISO), 500 g
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Xylose Lysine Deoxycholate (XLD) Agar (ISO) is a selective agar for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. Xylose Lysine Deoxycholate (XLD) Agar (ISO) is not intended for use in the diagnosis of disease or other conditions in humans. A selective, diagnostic agar for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. Originally formulated by Taylor to differentiate enteric pathogens, the agar is widely used as the preferred differential medium for Salmonella spp .For more information visit https://www.neogen.com/solutions/Microbiology/xylose-lysine-deoxycholate-xld-agar-iso
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NEOGEN Eosin Methylene Blue (EMB) Agar Levine, 500 g
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Eosin Methylene Blue Agar Levine is used for the isolation and differentiation of Gram-negative enteric bacilli and is not intended for use in the diagnosis of disease or other conditions in humans.This medium was introduced in 1916 by Holt-Harris and Teague to differentiate Escherichia spp. and Aerobacter spp. It was modified by Levine in 1918 who removed sucrose from the formula and increased the lactose content. The distinctive metallic sheen produced by E. coli on this medium is due to acid production resulting in an amide bonding between the eosin and methylene blue, other coliforms do not produce enough acid to cause this reaction. Eosin inhibits most Gram-positive organisms. The prepared medium is sensitive to light.For more information visit https://www.neogen.com/solutions/Microbiology/eosin-methylene-blue-agar-levine
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NEOGEN Rappaport Vassiliadis R10 Broth, 500 g
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Rappaport Vassiliadis R10 Broth is used for the selective enrichment of Salmonella spp. from foods in a laboratory setting. Rappaport Vassiliadis R10 Broth is not intended for use in the diagnosis of disease or other conditions in humans. Rappaport et al formulated an enrichment medium for Salmonella spp. that was modified by Vassiliadis et al. The Rappaport formulation, designated R 25/37°C, recommended incubation at 37°C. The Vassiliadis modification, designated R 10/43°C, had a reduced level of Malachite Green and recommended incubation at 43°C. Peterz later showed that incubation at 41.5 ± 0.5°C for 24 hours improved recovery of Salmonella spp. Rappaport-Vassiliadis R10 Broth is a selective enrichment medium that is used following pre-enrichment of the specimen.For more information visit https://www.neogen.com/solutions/Microbiology/rappaport-vassiliadis-r10-broth
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NEOGEN Vogel Johnson Agar, 500 g
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Vogel Johnson Agar is used for the isolation of staphylococci in a laboratory setting. Vogel Johnson Agar is not intended for use in the diagnosis of disease or other conditions in humans. Coagulase-positive staphylococci, primarily Staphylococcus aureus, are among the microorganisms that cause spoilage or chemical changes in cosmetic products. To isolate coagulase-positive, mannitol fermenting staphylococci, Vogel and Johnson modified Tellurite-Glycine Agar by Zebovitz, Evans, and Niven. The modification included increasing mannitol and adding a pH indicator. Vogel Johnson Agar selects and differentiates coagulase-positive staphylococci that ferment mannitol and reduce tellurite. Vogel Johnson (VJ) Agar is specified in standard methods testing for cosmetics and nutritional supplements.For more information visit https://www.neogen.com/solutions/Microbiology/vogel-johnson-agar
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Accurate Chemical Nycodenz Density Gradient Medium, Powder, Off-white, 500 g
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Nycodenz 500GM is a universal, non-ionic, and non-toxic density gradient medium designed for the high-resolution separation of biological particles. It comes as an off-white powder that forms stable low-osmolarity solutions. This product is ideal for isolating various cellular components without affecting their viability. Nycodenz has minimal interference with biochemical assays, making it an excellent choice for researchers seeking reliable separation methods.
- Form: Powder
- Color: Off-white
- Chemical Name: Iohexol
- Molecular Weight: 821 Da
- Density (Solid): 2.1 g/ml
- Maximum Solution Density: 1.426 g/ml (at 80% w/v)
- Solubility: Water, formamide, dimethyl formamide
- Stability: Up to 5 years
- Packaging Size: 500 g
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STEMCELL Technologies PneumaCult™-Ex Plus Medium
PneumaCult™-Ex Plus Medium is a defined, serum- and BPE-free cell culture medium that supports more expansion of primary human airway and nasal epithelial cells at each passage, compared to other commercially available expansion media. This medium also supports at least two additional passages of cell expansion with better differentiation potential, defined as the ability to form a pseudostratified mucociliary epithelium at the air-liquid interface (ALI) using PneumaCult-ALI Medium (Catalog #05001) or a cuboidal epithelium using PneumaCult-ALI-S Medium (Catalog #05050).PneumaCult-Ex Plus and either PneumaCult-ALI or PneumaCult-ALI-S constitute a fully integrated BPE-free culture system for in vitro human airway modeling. This robust and defined system is a valuable tool for basic respiratory research, toxicity studies, and drug development.
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Sciencell Research Laboratories CARDIAC MYOCYTE MEDIUM 500ML
Cardiac Myocyte medium is a complete medium designed for optimal growth of normal human cardiac myocytes in vitro. It is shipped as a kit with cardiac myocyte growth supplement and Pen/Strep solution.
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Research Products International Corp Nitschs' Medium with Vitamins, Powder, Makes 5 Liters
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Basal salt mixture containing micro and macro elements with vitamins as described by Nitsch & Nitsch (1969).
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Research Products International Corp Gamborg's B5 Medium, Powder, Makes 25 Liters
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Basal salt mixture containing micro and macro elements as described by Gamborg, et al. (1968). Package contains 3.05 grams of powder per liter of medium.
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